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Rabbit anti Human Adiponectin Receptor 1


Catalogue number: X1696P

Synonyms
Adiponectin receptor protein 1, Progestin and adipoQ receptor family member I, ADIPOR1, PAQR1,CGI-45

IsotypeN/A
Product Type Polyclonal Antibody
Units100 µg
HostRabbit
Species reactivity Human
Application Western Blot
Product Form
Unconjugated

Background
Alzheimer's disease (AD) is characterized by formation of plaques of amyloid beta peptide (Abeta). Autosomally-inherited AD had been shown only in connection with coding sequence mutations. Likely transcription factors whose mutation can cause loss of function are ADR1, MIG1, and PuF, and for gain of function; E12/E47, ITF-2, and RFX2. Adr1 controls the expression of genes required for ethanol, glycerol, and fatty acid utilization (in yeast). Adr1 can act directly on the promoters of ADH2, ACS1, GUT1, CTA1, and POT1. The yeast homolog of the AMP-activated protein kinase, Snf1, promotes Adr1 chromatin binding in the absence of glucose, and the protein phosphatase complex, Glc7.Reg1, represses its binding in the presence of glucose. A post-translational process is invloved in the regulation of Adr1 binding. Chromatin binding by Adr1 is not the only step in ADH2 transcription that is regulated by glucose repression, Adr1 can bind to chromatin in repressed conditions in the presence of hyperacetylated histones. In yeast, nuclear extracts prepared from glucose-repressed and glucose-derepressed cells are equally capable of supporting miniAdr1-dependent transcription and pre-initiation complex formation.

Positive Control
Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes.

Immunogen
Synthetic peptide near the N terminus of human Adiponectin Receptor 1 protein.

Purification Method
Ammonium Sulfate Precipitation

Concentration
See vial for concentration

Formulation
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis
Western Blotting

Applications
Antibody can be used for Western blotting (5-10µg/ml) and ELISA. Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Lahiri DK, Ge YW, Maloney B, Wavrant-De Vrieze F, Hardy J. ‘Characterization of two APP gene promoter polymorphisms that appear to influence risk of late-onset Alzheimer's disease.’ Neurobiol Aging. 2005 Nov-Dec;26(10):1329-41. Epub 2004 Dec 22. 2. Yamauchi T., Kamon J., Ito Y., Tsuchida A., Yokomizo T., Kita S., Sugiyama T., Miyagishi M., Hara K., Tsunoda M., Murakami K., Ohteki T., Uchida S., Takekawa S., Waki H., Tsuno N.H., Shibata Y., Terauchi Y., Froguel P., , Kadowaki T.; Cloning of adiponectin receptors that mediate antidiabetic metabolic effects.; Nature 423:762-769(2003).

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Rabbit anti Human Adiponectin Receptor 1

$355.00

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