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Sheep anti Rat ADAR1 (Adenosine Deaminase Acting on RNA 1)


Catalogue number: U110P

Synonyms
DRADA; double strandard RNA specific adenosine deaminase 1, ADAR1

IsotypeIgG
Product Type Antigen Immunoaffinity Purified Polyclonal
Units50 µg
HostSheep
Species reactivity Rat
Application Western Blot
Product Form
Affinity Purified

Background
Double stranded RNA specific adenosine deaminase 1 (ADAR1 or DRADA) is an enzyme involved in the editing of mammalian messenger RNAs by the site specific conversion of adenosine to inosine. ADAR 1 is thought to play a role in the editing of transcripts encoding non-NMDA glutamate receptor subunits, the 2C subtype of serotonin receptor, as well as hepatitis delta virus antigen.

Immunogen
GST fusion protein containing amino acid 1116-1176 of the rat DRADA coding sequence. The fusion protein was constructed in the pGEX-KG vector by inserting a fragment corresponding to nucleotides +3346 through +3525 of DRADA at the XbaI and XhoI sites in the vector.

Purification Method
Antigen Immunoaffiinity Purification

Concentration
See vial for concentration

Formulation
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis
Western Blotting

Applications
Applications: Immunoblotting - recommended dilution of 1:1000.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Room Temperature

Product Stability
Antibodies are stable for one year from purchase if stored frozen

References
1. Yang, J. -H., et al. 'Intracellular localization of differentially regulated RNA-specific adenosine deaminase isoforms in inflammation.' J. Biol. Chem. 2003, 278, 45833-45842 2. Wang, Q., et al. 'Stress-induced apoptosis associated with null mutation of ADAR1 RNA editing deaminase gene.' J. Biol. Chem. 2004, 279, 4952-4961 3. Yang, W., et al. 'ADAR1 RNA deaminase limits short interfering RNA efficacy in mammalian cells.' J. Biol. Chem. 2005, 280, 3946-3953

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Sheep anti Rat ADAR1 (Adenosine Deaminase Acting on RNA 1)

$249.00