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Rabbit anti Human ADAM 10 (CT) (kuz) (TNFa converting enzyme)


Catalogue number: X1106P

Synonyms
KUZ; MADM

IsotypeIgG
Product Type Polyclonal Antibody
Units100 µg
HostRabbit
Species reactivity Human
Mouse
Rat
Application Western Blot
Product Form
Unconjugated

Background
Proinflammatory cytokine tumor necrosis factor- (TNF- ) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF- , notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF- , notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Positive Control
Jurkat whole cell lysate

Immunogen
Synthetic peptide corresponding to amino acids 732 to 748 off the human ADAM10. Immungen sequence is identical in bovine and rat and differs from the mouse protein by one amino acid.

Purification Method
Antigen Immunoaffiinity Purification

Concentration
See vial for concentration

Formulation
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Functional Analysis
Western Blotting

Applications
Detects ADAM10 by Western blot at 0.25 to 1 µg/ml. Detects an 85 kDa band can be detected in Jurkat cell lysates which may represent precursor protein. A faint 60 kDa band is also detected in some cell lines, including Jurkat, which may be processed mature protein. Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Rosendahl, M.S., et al., Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases. J. Biol. Chem. 1997, 272, 24588-24593 2. Pan, D. & Rubin, G.M., Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis. Cell 1997, 90, 271-280 3. Qi, H., et al., Processing of the notch ligand delta by the metalloprotease Kuzbanian. Science 1999, 283, 91-94 4. Howard, L., et al., Molecular cloning of MADM: a catalytically active mammalian disintegrin-metalloprotease expressed in various cell types. Biochem. J. 1996, 317, 45-50

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
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Rabbit anti Human ADAM 10 (CT) (kuz) (TNFa converting enzyme)

$399.00

SKU: X1106P Categories: ,