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25(OH)Vitamin D3-1-alpha-Hydroxylase


Catalogue number: HYD001

Units100 µl
HostSheep
Application IHC
Immunohistochemistry
WB
Western Blotting
Background
The polyclonal antibody is well suited for the detection of 25(Hydroxy)Vitamin D3-1-alpha-Hydroxylase in tissues and tissue extracts e.g. in HKC-8 cell line from human proximal tubules (1). The seco-steroid hormone 1,25-Dihydroxyvitamin D3 plays a vital role in Calcium metabolism, in tissue differentiation and during the growth of bone. The biosynthesis of the hormone is catalysed by a mitochondrial P450-enzyme, 25-Hydroxy-Vitamin D3 1-alpha-Hydroxylase. Although the enzyme has been detected in different tissues, the main site of Vitamin D3 synthesis is the kidney. 25(OH)Vitamin D3-1-alpha-Hydroxylase, 56 kDa Protein in Western Blot.

Positive Control
Kidney

Immunogen
Synthetic peptide from the coding sequence of murine 25(OH)-Vitamin-D3-1-alpha-Hydroxylase (aa 266-289)

Purification Method
Sheep IgG fraction in glycine buffered NaCl solution pH 7.4; contains 0.1% sodium azide, 0.1% Epsilon-amino-n-caproic acid, 0.01% Benzamidine and 1 mM EDTA

Working Concentration
(liquid conc.) 1/500 (depending on fixation, species, unmasking method and secondary system)

Concentration
app. 7 mg/ml

Product
Sheep IgG fraction in glycine buffered NaCl solution pH 7.4; contains 0.1% sodium azide, 0.1% Epsilon-amino-n-caproic acid, 0.01% Benzamidine and 1 mM EDTA

Applications
WB, IHC

Cross Reactivity
Mouse, human

Storage
-20°C

References
1. Bland R, Walker EA, Hughes SV, Stewart PM, Hewison M. (1999) Constitutive expression of 25-hydroxyvitamin D3-1-alpha-hydroxylase in a transformed human proximal tubule cell line: Evidence for direct regulation of vitamin D metabolism by calcium. Endocrinology 140(5), 2027-2034. 2. Zehnder D, Bland R, Walker EA, Bradwell AR, Howie AJ, Hewison M, Stewart PM. (1999) Expression of 25-Hydroxyvitamin D3-1-alpha-hydoxylase in human kidney. J. Am. Soc. Nephrol. 10; 2465-2473 3. Bland R, Markovic D, Hills CE, Hughes SV, Chan SL, Squires PE, Hewison M (2004) Expression of 25-hydroxyvitamin D3-1alpha-hydroxylase in pancreatic islets. J Steroid Biochem Mol Biol. 89-90(1-5):121-125. 4. Takeyama K, Kitanaka S, Sato T, Kobori M, Yanagisawa J, Kato S. (1997) 25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis. Science;277(5333):1827-30 (cloning of 1-alpha-Hydroxylase)

Caution
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Safety Datasheet(s) for this product:

25(OH)Vitamin D3-1-alpha-Hydroxylase

$474.00

SKU: HYD001 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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