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25(OH)Vitamin D3-1-alpha-Hydroxylase

Catalogue number: HYD001

Units100 µl
Application Immunohistochemistry
Western Blotting

The polyclonal antibody is well suited for the detection of 25(Hydroxy)Vitamin D3-1-alpha-Hydroxylase in tissues and tissue extracts e.g. in HKC-8 cell line from human proximal tubules (1). The seco-steroid hormone 1,25-Dihydroxyvitamin D3 plays a vital role in Calcium metabolism, in tissue differentiation and during the growth of bone. The biosynthesis of the hormone is catalysed by a mitochondrial P450-enzyme, 25-Hydroxy-Vitamin D3 1-alpha-Hydroxylase. Although the enzyme has been detected in different tissues, the main site of Vitamin D3 synthesis is the kidney. 25(OH)Vitamin D3-1-alpha-Hydroxylase, 56 kDa Protein in Western Blot.


Immunogen: Synthetic peptide from the coding sequence of murine 25(OH)-Vitamin-D3-1-alpha-Hydroxylase (aa 266-289)

Sheep IgG fraction in glycine buffered NaCl solution pH 7.4; contains 0.1% sodium azide, 0.1% Epsilon-amino-n-caproic acid, 0.01% Benzamidine and 1 mM EDTA

Purification Method: Sheep IgG fraction in glycine buffered NaCl solution pH 7.4; contains 0.1% sodium azide, 0.1% Epsilon-amino-n-caproic acid, 0.01% Benzamidine and 1 mM EDTA

Concentration: app. 7 mg/ml


Species Reactivity: Mouse, human


Working Concentration: (liquid conc.) 1/500 (depending on fixation, species, unmasking method and secondary system)

Positive Control: Kidney


This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

1. Bland R, Walker EA, Hughes SV, Stewart PM, Hewison M. (1999) Constitutive expression of 25-hydroxyvitamin D3-1-alpha-hydroxylase in a transformed human proximal tubule cell line: Evidence for direct regulation of vitamin D metabolism by calcium. Endocrinology 140(5), 2027-2034. 2. Zehnder D, Bland R, Walker EA, Bradwell AR, Howie AJ, Hewison M, Stewart PM. (1999) Expression of 25-Hydroxyvitamin D3-1-alpha-hydoxylase in human kidney. J. Am. Soc. Nephrol. 10; 2465-2473 3. Bland R, Markovic D, Hills CE, Hughes SV, Chan SL, Squires PE, Hewison M (2004) Expression of 25-hydroxyvitamin D3-1alpha-hydroxylase in pancreatic islets. J Steroid Biochem Mol Biol. 89-90(1-5):121-125. 4. Takeyama K, Kitanaka S, Sato T, Kobori M, Yanagisawa J, Kato S. (1997) 25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis. Science;277(5333):1827-30 (cloning of 1-alpha-Hydroxylase)

Safety Datasheet(s) for this product:
EA_Sodium Azide
/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf

25(OH)Vitamin D3-1-alpha-Hydroxylase


SKU: HYD001 Categories: ,


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Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370