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Mouse anti Human 14-3-3 theta/tau


Catalogue number: X1010

Synonyms
mouse anti 14-3-3 theta/tau, theta tau

Clone3B9
IsotypeIgG1
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Species reactivity Bovine
Human
Mouse
Rat
Application Enzyme Immunoassay
Western Blot
Product Form
Unconjugated

Background
At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and, through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

Positive Control
3T3, PC12, Jurkat

Immunogen
Recombinant 14-3-3 / protein

Purification Method
Protein A/G Chromatography

Concentration
See vial for concentration

Formulation
Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5 and 3 mM sodium azide

Functional Analysis
Western Blotting

Applications
Clone 3B9 anti-14-3-3θ/τ reacts specifically with the θ/τ isoform and does not react with 14-3-3ζ .Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Room Temperature

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Umahara, T., et al. 'Structure-oriented review of 14-3-3 protein isoforms in geriatric neuroscience.' Geriatr. Gerontol. Int. 2012, epub. 2. Choi, M.R., et al. 'Effect of fluoxetine on the expression of tryptophan hydroxylase and 14-3-3 protein in the dorsal raphe nucleus and hippocampus of rat.' J. Chem. Neuronat., 2012, 43, 96-102 3. Obsil, T., et al. 'Structural basis of 14-3-3 protein function.' Semin. Cell Dev. Biol. 2011, 22, 663-672.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Glycerol
Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-glycerolV2.pdf
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Mouse anti Human 14-3-3 theta/tau

$201.00

SKU: X1010 Categories: ,