Mouse anti Human 14-3-3 theta/tauCatalogue number: X1010
|Product Type||Monoclonal Antibody|
At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and, through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.
Synonyms: mouse anti 14-3-3 theta/tau, theta tau
Immunogen: Recombinant 14-3-3 / protein
Product Form: Unconjugated
Formulation: Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5 and 3 mM sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Clone 3B9 anti-14-3-3?/? reacts specifically with the ?/? isoform and does not react with 14-3-3? .Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: 3T3, PC12, Jurkat
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Ship Conditions: Room Temperature
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
1. Umahara, T., et al. 'Structure-oriented review of 14-3-3 protein isoforms in geriatric neuroscience.' Geriatr. Gerontol. Int. 2012, epub. 2. Choi, M.R., et al. 'Effect of fluoxetine on the expression of tryptophan hydroxylase and 14-3-3 protein in the dorsal raphe nucleus and hippocampus of rat.' J. Chem. Neuronat., 2012, 43, 96-102 3. Obsil, T., et al. 'Structural basis of 14-3-3 protein function.' Semin. Cell Dev. Biol. 2011, 22, 663-672.
Database Name: UniProt
Accession number: P27348
Species Accession: Human
Safety Datasheet(s) for this product:
Mouse anti Human 14-3-3 theta/tau