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Mouse anti Human 14-3-3 theta/tau

Catalogue number: X1010

Clone3B9
IsotypeIgG1
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Species reactivity Bovine
Human
Mouse
Rat
Application Enzyme Immunoassay
Western Blot

Background
At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and, through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

Synonyms: mouse anti 14-3-3 theta/tau, theta tau

Source

Immunogen: Recombinant 14-3-3 / protein

Product

Product Form: Unconjugated

Formulation: Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5 and 3 mM sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Clone 3B9 anti-14-3-3?/? reacts specifically with the ?/? isoform and does not react with 14-3-3? .Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: 3T3, PC12, Jurkat

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Ship Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

References
1. Umahara, T., et al. 'Structure-oriented review of 14-3-3 protein isoforms in geriatric neuroscience.' Geriatr. Gerontol. Int. 2012, epub. 2. Choi, M.R., et al. 'Effect of fluoxetine on the expression of tryptophan hydroxylase and 14-3-3 protein in the dorsal raphe nucleus and hippocampus of rat.' J. Chem. Neuronat., 2012, 43, 96-102 3. Obsil, T., et al. 'Structural basis of 14-3-3 protein function.' Semin. Cell Dev. Biol. 2011, 22, 663-672.

Protein Reference(s)

Database Name: UniProt

Accession number: P27348

Species Accession: Human


Safety Datasheet(s) for this product:
Glycerol
Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-glycerolV2.pdf
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Mouse anti Human 14-3-3 theta/tau

$254.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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