Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Chicken anti Indole-3-butyric acid

  • Product Code: T0158p
  • Size: 1 ml
  • Availability: In Stock In Stock
  • Price (USD): $455

Product Code

T0158p		 Quantity:      

Data Sheet

Product Name

Chicken anti Indole-3-butyric acid

Size

1 ml

Price (USD)

$455

Background

Indole-3-butyric acid (IBA) is a plant hormone belonging to the group of auxins. It is used to stimulate root formation in plant cuttings.

Clone Name

Polyclonal

Host

Chicken

Product Usage

Cross Reactivity Indole-3-aceteic acid < 0.1 %

Product Type

Primary Antibodies

Applications

The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Samples may include cutting medium samples and extraction of these samples may be performed using PBS-10% methanol, followed by neutralisation of the extract. Suggested dilution 1:12,800 in indirect ELISA (this titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Plates are coated with 400 ng/ml ovalbumine-conjugated indole-3-butyric acid. HRP-conjugated anti-chicken IgY should be used as a tracer in a 1:8,000 dilution.

Fields of Interest

Toxic Compounds

Product Formulation

Each vial contains 1 ml extract purified (PEG) chicken polyclonal antibody.

Storage Conditions

Store at 4