Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Rabbit anti Cat IgG (heavy and light chains), conjugated with FITC

  • Product Code: RACa-IgG(H+L)-FITC
  • Size: 2 ml
  • Price (USD): $393

Product Code

RACa-IgG(H+L)-FITC		 Quantity:      

Product Name

Rabbit anti Cat IgG (heavy and light chains), conjugated with FITC

Size

2 ml

Price (USD)

$393

Background

The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in cat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of cat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Host

Rabbit

Species Reactivity

Feline

Product Usage

Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Cat IgG, heavy and light chains.

Product Type

Secondary Antibodies

Applications

ELISA,Immunocytochemistry,Immunohistochemistry (frozen),Dot blot,Immunoblotting

Fields of Interest

Veterinary

Product Source

Purified normal IgG isolated from pooled cat serum. Freund

Product Formulation

FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.

Product Specificity

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion with the following results: Bovine - Chicken - Dog + Duck - ferret + Goat -Guinea Pig

Storage Conditions

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4