Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

CD15/ Lewis X antigen/ alpha-(1,3)-fucosyltransferase 4

  • Product Code: MUB0367P
  • Size: 0.1 mg
  • Availability: In Stock In Stock
  • Price (USD): $310

Product Code

MUB0367P		 Quantity:      

Data Sheet

Product Name

CD15/ Lewis X antigen/ alpha-(1,3)-fucosyltransferase 4

Size

0.1 mg

Price (USD)

$310

Background

CD15 is a carbohydrate that is referred to as the Lewis X antigen, also known as alpha-(1,3)-fucosyltransferase 4, which is expressed on a variety of glycolipids and glycoproteins found on the cell surface of neutrophils, monocytes, eosinophils and non-hematopoietic tissues including embryonic tissues. CD15 is reported to function as an adhesion molecule and is involved in chemotaxis and phagocytosis. Expression of CD15 is also associated with a wide variety of tumour cells including some adenocarcinomas and certain types of myeloid and lymphoid leukaemias. Reed-Sternberg cells, found in cases of Hodgkins Lymphoma, also express CD15 and antibodies recognising the CD15 antigen are often used to help differentiate between Hodgkins and non-Hodgkins lymphoma.

Clone Name

BRA4F1

Host

Mouse

Isotype

IgM

Species Reactivity

Human

Product Type

Primary Antibodies

Applications

The BRA4F1 antibody is suitable for the detection of CD15 by flow cytometry, immunocytochemisty and immunohistochemistry of frozen and paraffin embedded tissues. Optimal antibody dilutions for the different applications should be determined by titration.

Fields of Interest

CD markers, Cancer, Cell adhesion, Immunology

Product Formulation

Each vial contains 100

Storage Conditions

Store at 4oC, or in small aliquots at -20oC.

References

Chorv