CD15 is a carbohydrate that is referred to as the Lewis X antigen, also known as alpha-(1,3)-fucosyltransferase 4, which is expressed on a variety of glycolipids and glycoproteins found on the cell surface of neutrophils, monocytes, eosinophils and non-hematopoietic tissues including embryonic tissues. CD15 is reported to function as an adhesion molecule and is involved in chemotaxis and phagocytosis. Expression of CD15 is also associated with a wide variety of tumour cells including some adenocarcinomas and certain types of myeloid and lymphoid leukaemias. Reed-Sternberg cells, found in cases of Hodgkins Lymphoma, also express CD15 and antibodies recognising the CD15 antigen are often used to help differentiate between Hodgkins and non-Hodgkins lymphoma.
The antibody BRA4F1 is directed against human CD15. The antibody does not cross react with the sialylated form of CD15 (CD15s).
Flow cytometry, Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Immunocytochemistry
Fields of Interest
CD markers, Cancer, Cell adhesion, Immunology
BRA4F1 is a mouse IgM monoclonal antibody derived from the fusion of SP2.0-Ag14 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with K562 cells.
Each vial contains 100 µl 1mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. The product is delivered in lyophilised format.
Store at 4oC, or in small aliquots at -20oC.