Members of the tumor necrosis factor receptor (TNFR) family are emerging as important molecules impliCated in the regulation of prolifeRation, differentiation and survival of T and B lymphocytes. Among these receptors is CD27, the function of which has thus far only been studied in the Human system, where it amplifies the T cell prolifeRative response induced by TCR triggering. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for geneRation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-_B and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a pro-apoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
LG.3A10 and LG.8A6 see a different epitope. A combination can therefore be developed into a double determinant ELISA to detect soluble Mouse CD27. LG.3A10 cross-react with Human CD27, LG.8A6 does not.
ELISA,Flow Cytometry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Immunoprecipitation
Fields of Interest
mAbs to murine CD27 were geneRated injecting a mixture of the mCD27tag expresssing clones AR0524 and AR0530; Hamster spleen cells and SP2/0 murine myeloma cells were fused.
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Store at 4¡C, or in small aliquots at -20¡C.