The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:10 and 1:30.
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion anti several sera with the following results:
cow - Duck - Horse - Rat + Cat - Guinea Pig + Monkey - Sheep - Chicken - Human - pigeon - Swine - Dog - Hamster
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
Fields of Interest
TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4