Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Goat anti Mouse IgG3 (subclass specific), conjugated with Horseradish peroxidase

  • Product Code: GAM-IgG3-PO
  • Size: 1 ml
  • Price (USD): $453

Product Code

GAM-IgG3-PO		 Quantity:      

Product Name

Goat anti Mouse IgG3 (subclass specific), conjugated with Horseradish peroxidase

Size

1 ml

Price (USD)

$453

Background

The reactivity of the antiserum is directed to the subclass IgG3. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:7,000 depending on the method used.

Host

Goat

Species Reactivity

Mouse

Product Usage

Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse IgG3, subclass specific.

Product Type

Secondary Antibodies

Applications

ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting

Fields of Interest

Veterinary

Product Source

Pools of purified homogenous IgG3 isolated from pooled mouse serum. Freund

Product Formulation

Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Product Specificity

This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. However this conjugate has been passed over appropriate immunoadsorbents to remove antibodies cross-reacting with Human immunoglobulins. This renders it specific for use in test systems containing material of Human origin (e.g. Human tissue/Mouse monoclonal antibody to a Human tissue constituent/anti Mouse Ig isotype-specific immunoconjugate.

Storage Conditions

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4