The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal dog serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in dog serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgM from serum or plasma.
Precipitating polyclonal Goat antiSerum to Dog IgM, Fc specific
Fields of Interest
Highly purified normal IgM isolated from pooled dog serum. Freund
Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
The antiSerum does not cross-react with any other component of the Dog Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4