Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Goat anti Chicken IgA (Fc specific), conjugated with Horseradish peroxidase

  • Product Code: GACh-IgA(Fc)-PO
  • Size: 1 ml
  • Price (USD): $330

Product Code

GACh-IgA(Fc)-PO		 Quantity:      

Product Name

Goat anti Chicken IgA (Fc specific), conjugated with Horseradish peroxidase

Size

1 ml

Price (USD)

$330

Background

The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in chicken serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in chicken serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Host

Goat

Species Reactivity

Chicken

Product Usage

Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Chicken IgA, Fc specific

Product Type

Secondary Antibodies

Applications

ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting

Fields of Interest

Veterinary

Product Source

Purified normal IgA isolated from pooled chicken serum. Freund

Product Formulation

Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Product Specificity

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Storage Conditions

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4