Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Goat anti Chicken IgA (Fc specific), conjugated with FITC

  • Product Code: GACh-IgA(Fc)-FITC
  • Size: 1 ml
  • Price (USD): $330

Product Code

GACh-IgA(Fc)-FITC		 Quantity:      

Product Name

Goat anti Chicken IgA (Fc specific), conjugated with FITC

Size

1 ml

Price (USD)

$330

Background

The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against chicken serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgA used as immunogens. It does not react with IgG, IgG/Fab fragments and IgM or any non-Ig protein in chicken serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used.

Host

Goat

Species Reactivity

Chicken

Product Usage

Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Chicken IgA Fc specific

Product Type

Secondary Antibodies

Applications

ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence

Fields of Interest

Veterinary

Product Source

Purified IgA isolated from chicken serum. Freund

Product Formulation

FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Product Specificity

This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this antiSerum has not been tested in detail.

Storage Conditions

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4