The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in chicken serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user
IgG fraction of polyclonal Goat antiSerum to Chicken IgA, Fc specific
Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
Fields of Interest
Purified normal IgA isolated from pooled chicken serum. Freund
Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4