Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Goat anti Cat serum proteins

  • Product Code: GACa-TSP
  • Size: 1 ml
  • Price (USD): $208

Product Code

GACa-TSP		 Quantity:      

Product Name

Goat anti Cat serum proteins

Size

1 ml

Price (USD)

$208

Background

In immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipitation can be observed of not less than 16 individual proteins components. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence or absence of an individual component. To evaluate the purity of an isolated serum protein including immunoglobulins. Since immunoprecipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar Nordic Nr. 2 contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar (Agar Nordic nr. 1) with low electroendosmosis favours the resolution of the proteins in the alpha regions, while the major components in the beta-gamma region can still be identified.

Host

Goat

Species Reactivity

Feline

Product Usage

Precipitating polyclonal Goat antiSerum to Cat Serum proteins

Product Type

Primary Antibodies

Applications

Immunoprecipitation

Fields of Interest

Veterinary

Product Source

Pooled whole cat serum and partly purified serum fractions. FreundÕs complete adjuvant is used in the first step of the immunization procedure.

Product Formulation

Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.

Product Specificity

Inter-species cross-reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiSerum has not been adsorbed for such cross-reactivity. Consequently it is not species-specific.

Storage Conditions

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4¡C; prolonged storage at or below -20¡C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4¡C, not refrozen, and preferably used the same day. Lyophilized at +4¡ C--at least 10 years. Reconstituted at or below -20¡ C--3-5 years. Reconstituted at +4¡ C--7 days