Tested in immunoelectrophoresis and double radial immunodiffusion against pooled serum precipitation of at least 12 individual proteins have been observed. Direct fluorescent staining of bovine serum proteins at the cellular and subcellular level of appropriately treated cell and tissue substrates.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Bovine total Serum proteins
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
Fields of Interest
Pooled whole serum and selected protein fractions isolated from pooled bovine serum. Freund
FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4