The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in goat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of goat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions fir histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable no-precipitating antibody-binding assays between 1:2,000 and 1:15,000.
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results:
bovine ++ guinea pig
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
Fields of Interest
Horseradish peroxidase-coupled purified hyperimmune donkey IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4