Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Donkey anti Goat IgG (heavy and light chains)

  • Product Code: DoAG/IgG(H+L)
  • Size: 1 ml
  • Availability: In Stock In Stock
  • Price (USD): $125

Product Code

DoAG/IgG(H+L)		 Quantity:      

Data Sheet

Product Name

Donkey anti Goat IgG (heavy and light chains)

Size

1 ml

Price (USD)

$125

Background

The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses, IgG1 and IgG2. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against goat serum a single characteristic precipitin line with a spur is usually obtained, representing primarily IgG2. If the IgG1 level in the sample approaches the concentration of IgG2, it may become visible as a second precipitin line. It does not react with any non-Ig protein in goat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal goat serum; to prepare an immunoadsorbent for the production of Ig-free goat serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.

Clone Name

Polyclonal

Host

Donkey

Species Reactivity

Caprine

Product Usage

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results: bovine ++ guinea pig

Product Type

Secondary Antibodies

Applications

Immunoprecipitation. In immunoelectrophoresis use 2 _l or equivalent against 120 _l antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 _l antiserum in a 3 mm diameter centre well and 2 _l serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitin titre not less than 1:128 when tested against normal goat serum in agar block titration.

Fields of Interest

Veterinary

Product Formulation

Delipidated, heat inactivated lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled donkey serum. No foreign proteins added.

Storage Conditions

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4