Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Rabbit anti-native Green Fluorescent Protein (Immunoaffinity purified)

  • Product Code: Bii-nGFPab4-25
  • Size: 25 ug
  • Price (USD): $99

Product Code

Bii-nGFPab4-25		 Quantity:      

Product Name

Rabbit anti-native Green Fluorescent Protein (Immunoaffinity purified)

Size

25 ug

Price (USD)

$99

Background

Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research, embryology, and plant sciences, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols. The antibody is also suitable for immunocytochemical detection of proteins labeled with the different variants of GFP.

Host

Rabbit

Isotype

Polyclonal Ig

Species Reactivity

n/a

Product Usage

The GFP antiserum is directed against native green fluorescent protein from A.victoria jellyfish. The antibody recognises not only the native GFP, but also recombinant protein (E.Coli), native GFP-fusion proteins and all variants of GFP tested. Cross reactivity with E.coli. proteins is minimal.

Product Type

Primary Antibodies

Applications

Western blotting, ELISA and Immunocytochemistry.

Product Source

The anti-GFP antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with highly purified native GFP derived from Aequorea victoria jellyfish.

Product Formulation

Each vial contains 25ug 1 mg/ml immunoaffinity purified polyclonal antiserum in 10 mM TRIS buffer pH 8.0, containing 0.02% sodium azide

Product Specificity

Aequoria Victoria (native) GFP, recombinant (E.Coli) and native GFP-fusion proteins, and all variants of GFP tested.

Storage Conditions

Store at 4oC, or in small aliquots at -20oC for storage during longer periods.