Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Image Library

5-HT5A Receptor (HTR5A)
(Cat#X2106P)

5-HT5A Receptor  staining of formallin fixed paraffin embedded human brain (cortex) tissue at a dilution of 5 ug/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

5-HT5A Receptor staining of formallin fixed paraffin embedded human brain (cortex) tissue at a dilu..More

5-HT5A Receptor (HTR5A)
(Cat#X2110P)

5-HT5A Receptor staining of formallin fixed paraffin embedded human Brain (Cortex) tissue at a dilution of 10 µg/mll. Antigen retrieval using a citrate buffer and steam/heat was utilized.

5-HT5A Receptor staining of formallin fixed paraffin embedded human Brain (Cortex) tissue at a dilut..More

5-HT5A Receptor (HTR5A)
(Cat#X2142P)

HTR5A staining of formallin fixed paraffin embedded human brain (cerebellum) tissue at a dilution of 10 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

HTR5A staining of formallin fixed paraffin embedded human brain (cerebellum) tissue at a dilution of..More

5-HT7 Receptor (HTR7)
(Cat#X2140P)

HTR7 staining of formallin fixed paraffin embedded human brain (cortex) tissue at a dilution of 10 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

HTR7 staining of formallin fixed paraffin embedded human brain (cortex) tissue at a dilution of 10 &..More

5-HT7 Receptor (HTR7)
(Cat#X2141P)

HTR7 staining of formallin fixed paraffin embedded human brain (cortex) tissue at a dilution of 10 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

HTR7 staining of formallin fixed paraffin embedded human brain (cortex) tissue at a dilution of 10 &..More

5-HT7 Receptor (HTR7)
(Cat#X2152P)

HTR7 staining of formallin fixed paraffin embedded human brain (cerebellum) tissue at a dilution of 10 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

HTR7 staining of formallin fixed paraffin embedded human brain (cerebellum) tissue at a dilution of ..More

5T4 Oncofetal Trophoblast glycoprotein
(Cat#X2738P)

Immunohistochemical staining of normal human kidney tissue using Sirt1 antibody (Cat. No. X2739P) at 10 µg/ml and detected using anti-Rabbit HRP secondary antibody and visualized using DAB substrate and hematoxylin counterstain.

Immunohistochemical staining of normal human kidney tissue using Sirt1 antibody (Cat. No. X2739P) at..More

Acid Ceramidase 1
(Cat#X2668P)

Immunohistochemical staining of human heart tissue using Acid Ceramidase 1 antibody at 10 µg/ml.

Immunohistochemical staining of human heart tissue using Acid Ceramidase 1 antibody at 10 µg/m..More

Actin, (cytoplasmic 1) (NT)
(Cat#X2742P)

Immunohistochemistry image using beta-Actin (NT) antibody (Cat. No. X2742P) on normal human liver tissue sections at 10

Immunohistochemistry image using beta-Actin (NT) antibody (Cat. No. X2742P) on normal human liver ti..More

Actin, cytoplasmic 1 (NT)
(Cat#X2408P)

Immunohistochemistry staining of normal human liver tissue using Actin antibody (Cat. No. X2408P) at 10 µg/ml.

Immunohistochemistry staining of normal human liver tissue using Actin antibody (Cat. No. X2408P) at..More

ADAM DEC1
(Cat#X2416P)

Staining on paraffin embedded normal human tonsil sections. Primary Ab (IgG cut or immunoaffinity purified) at 10 µg/ml. Antigen retrieval used: 10 mM Na Citrate pH 6.0, 10 minutes pressure cooker method., developed with anti rabbit HRP and DAP substrate. Counterstained with methyl blue.

Staining on paraffin embedded normal human tonsil sections. Primary Ab (IgG cut or immunoaffinity pu..More

ADAM DEC1
(Cat#X2727P)

Staining of human tonsil sections with ADAM-DEC1 antibody (Cat. No. X2727P) at 2 µg/ml.

Staining of human tonsil sections with ADAM-DEC1 antibody (Cat. No. X2727P) at 2 µg/ml.

ADAR2 (Adenosine Deaminase Acting on RNA 2)
(Cat#U115P)

Immunofluorescence staining of mouse anterior horns of spinal cord at 6 months, 15 months and 26 months of age.  Antibody used at 1:100 dilution and visualized using donkey anti-sheep conjugated to Alexa 555 dye (red color).  The blue color is a fluorescent nuclear probe, TOPRO3.

Immunofluorescence staining of mouse anterior horns of spinal cord at 6 months, 15 months and 26 mon..More

Ah Receptor (AHR)
(Cat#X2250P)

AHR staining of formallin fixed paraffin embedded respiratory epithelium and bronchial smooth muscle tissue at a dilution of 6 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

AHR staining of formallin fixed paraffin embedded respiratory epithelium and bronchial smooth muscle..More

Ah Receptor (AHR)
(Cat#X2251P)

AHR staining of formallin fixed paraffin embedded respiratory epithelium and bronchial smooth muscle tissue at a dilution of 13 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

AHR staining of formallin fixed paraffin embedded respiratory epithelium and bronchial smooth muscle..More

Ah Receptor (AHR)
(Cat#X2252P)

AHR staining of formallin fixed paraffin embedded respiratory epithelium and bronchial smooth muscle tissue at a dilution of 6 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

AHR staining of formallin fixed paraffin embedded respiratory epithelium and bronchial smooth muscle..More

Ah Receptor (AHR)
(Cat#X2253P)

AHR staining of formallin fixed paraffin embedded respiratory epithelium and bronchial smooth muscle tissue at a dilution of 17 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

AHR staining of formallin fixed paraffin embedded respiratory epithelium and bronchial smooth muscle..More

Akt/PKB [pS473]
(Cat#X2010M)

Immunofluorescence Staining Serum-starved NIH3T3 cells left untreated (left) or treated with PDGF (right) were fixed prior to immunostaining with the Akt [pS473] rabbit monoclonal antibody. The signal was detected with an anti-rabbit FITC conjugated secondary antibody. The data show that the antibody detected phosphorylated Akt in PDGF-treated NIH3T3 cells. Cells were counterstained with DAPI. Please visit our website (biosource.com) to view the images in full color.

Immunofluorescence Staining Serum-starved NIH3T3 cells left untreated (left) or treated with PDGF (r..More

APM2 (CT)
(Cat#X2385P)

Immunohistochemical staining of normal human liver tissue using APM2 antibody (Cat. No. X2385P) at 15 µg/ml.

Immunohistochemical staining of normal human liver tissue using APM2 antibody (Cat. No. X2385P) at 1..More

Aprataxin
(Cat#X2411P)

Immunohistochemical staining of human lung tissue using Aprataxin antibody at 5 µg/ml

Immunohistochemical staining of human lung tissue using Aprataxin antibody at 5 µg/ml

Aprataxin
(Cat#X2725P)

Immunohistochemical staining of normal human lung tissue using Aprataxin antibody (Cat. No. X2725P) at 10 µg/ml.

Immunohistochemical staining of normal human lung tissue using Aprataxin antibody (Cat. No. X2725P) ..More

ATIC
(Cat#X2062M)

Immunohistochemical staining of normal colon tissue and colon carcinoma tissue using ATIC antibody (Cat. No. X2062M).

Immunohistochemical staining of normal colon tissue and colon carcinoma tissue using ATIC antibody (..More

ATP-Binding Cassette, Sub-Family B (Mdr/Tap), Member 1 (Abcb1)
(Cat#X2192P)

Abcb1 staining of formallin fixed paraffin embedded adrenal, cortex tissue at a dilution of 20 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

Abcb1 staining of formallin fixed paraffin embedded adrenal, cortex tissue at a dilution of 20 &micr..More

Attractin (ATRN)
(Cat#X2201P)

ATRN staining of formallin fixed paraffin embedded spleen tissue at a dilution of 20 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

ATRN staining of formallin fixed paraffin embedded spleen tissue at a dilution of 20 µg/ml. An..More

Attractin (ATRN)
(Cat#X2202P)

ATRN staining of formallin fixed paraffin embedded spleen tissue at a dilution of 20 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

ATRN staining of formallin fixed paraffin embedded spleen tissue at a dilution of 20 µg/ml. An..More

BCRP (ATP-binding cassette sub-family G member 2)
(Cat#X2731P)

Immunohistochemical staining of normal human testis tissue using BCRP antibody (Cat. No. X2731P) at 10 µg/ml and detected using anti-Rabbit HRP secondary antibody and visualized using DAB substrate and hematoxylin counterstain.

Immunohistochemical staining of normal human testis tissue using BCRP antibody (Cat. No. X2731P) at ..More

Beta-site APP-Cleaving Enzyme 1 (BACE1)
(Cat#X2230P)

BACE1 staining of formallin fixed paraffin embedded pancreas tissue at a dilution of 2.5 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

BACE1 staining of formallin fixed paraffin embedded pancreas tissue at a dilution of 2.5 µg/ml..More

Beta-site APP-Cleaving Enzyme 1 (BACE1)
(Cat#X2234P)

BACE1 staining of formallin fixed paraffin embedded human brain, alzheimers tissue at a dilution of 3 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

BACE1 staining of formallin fixed paraffin embedded human brain, alzheimers tissue at a dilution of ..More

Bombesin Receptor Subtype 3 (BRS3)
(Cat#X2111P)

Bombesin Receptor Subtype 3 (BRS3) staining of formallin fixed paraffin embedded human Pancreas, Islet tissue at a dilution of 10 µg/mll. Antigen retrieval using a citrate buffer and steam/heat was utilized.

Bombesin Receptor Subtype 3 (BRS3) staining of formallin fixed paraffin embedded human Pancreas, Isl..More

Bombesin Receptor Subtype 3 (BRS3)
(Cat#X2128P)

BRS3 staining of formallin fixed paraffin embedded uterus (pregnant) tissue at a dilution of 10 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

BRS3 staining of formallin fixed paraffin embedded uterus (pregnant) tissue at a dilution of 10 &mic..More

Brain-Specific Angiogenesis Inhibitor 1 (BAI1)
(Cat#X2143P)

BAI1 staining of formallin fixed paraffin embedded human brain tissue at a dilution of 20 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

BAI1 staining of formallin fixed paraffin embedded human brain tissue at a dilution of 20 µg/m..More

BrdU (bromodeoxyuridine)
(Cat#X1028)

Immunohistochemical staining using BrdU antbody (Cat. No. X1028) on mouse intestine from mice treated with BrdU.

Immunohistochemical staining using BrdU antbody (Cat. No. X1028) on mouse intestine from mice treate..More

Calcium Channel
(Cat#X2391P)

Immunohistochemical staining of normal human brain tissue using alpha1A calcium channel antibody (Cat. No. X2391P) at 15 µg/ml

Immunohistochemical staining of normal human brain tissue using alpha1A calcium channel antibody (Ca..More

Calcium Channel
(Cat#X2713P)

Immunohistochemical staining of normal human kidney tissue with alpha1C calcium channel antibody (Cat. No. X2713P) at 15 µg/ml

Immunohistochemical staining of normal human kidney tissue with alpha1C calcium channel antibody (Ca..More

Calcium Channel
(Cat#X2715P)

Immunohistochemical staining of human melanoma tumor tissue using alpha1G calcium channel antibody (Cat. No. X2715P) at 15 µg/ml.

Immunohistochemical staining of human melanoma tumor tissue using alpha1G calcium channel antibody (..More

Calcium Homeostasis Modulator Protein 1
(Cat#X2723P)

Staining on paraffin embedded human hepatocellular carcinoma sections. Primary Ab (immunoaffinity purified) at 10 µg/ml. Antigen retrieval used: 10 mM Na Citrate pH 6.0, 10 minutes pressure cooker method, developed with anti rabbit HRP and DAP substrate.  Counterstained with methyl blue.

Staining on paraffin embedded human hepatocellular carcinoma sections. Primary Ab (immunoaffinity pu..More

Calreticulin
(Cat#Z100P)

Immunofluorescence of MCF-7 cells stained with antibody at 1:50 dilution.

Immunofluorescence of MCF-7 cells stained with antibody at 1:50 dilution.

Caveolin-1 (CAV1)
(Cat#X2254P)

CAV1 staining of formallin fixed paraffin embedded respiratory epithelium bronchial smooth muscle, and Alveoli tissue at a dilution of 3 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

CAV1 staining of formallin fixed paraffin embedded respiratory epithelium bronchial smooth muscle, a..More

Caveolin-1 (CAV1)
(Cat#X2255P)

CAV1 staining of formallin fixed paraffin embedded respiratory epithelium and Alveoli tissue at a dilution of 4 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

CAV1 staining of formallin fixed paraffin embedded respiratory epithelium and Alveoli tissue at a di..More

Caveolin-1 (CAV1)
(Cat#X2256P)

CAV1 staining of formallin fixed paraffin embedded respiratory epithelium, bronchial smooth muscle, and Alveoli tissue at a dilution of 17 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

CAV1 staining of formallin fixed paraffin embedded respiratory epithelium, bronchial smooth muscle, ..More

CCR10 (G-protein-Coupled Receptor GPR2)
(Cat#X2094P)

CCR10 staining of formallin fixed paraffin embedded human skin tissue at a dilution of 5ug/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

CCR10 staining of formallin fixed paraffin embedded human skin tissue at a dilution of 5ug/ml. Antig..More

CD15-NA FITC
(Cat#X1496M)

Immunofluorescence staining using CD15-FITC IgG3 antibody (Cat. No. X1496M) on human neutrophils.

Immunofluorescence staining using CD15-FITC IgG3 antibody (Cat. No. X1496M) on human neutrophils.

CD56
(Cat#0561)

Formalin-fixed, paraffin-embedded human neuroblastoma stained with Exalpha's CD56, clone C5.9 at 2 ug/ml using peroxidase-conjugate and DAP chromogen. Cell membrane staining of tumor cells is clearly evident. High-temperature heating was used as the retrieval protocol, pressure cooker with citrate buffer, pH 6.5, for 10 min. Exalpha?s anti-CD56,clone C5.9, was added at 2 ug/ml for 1 hour at room temperature followed by anti-Mouse HRP for 30 minutes at room temperature. Staining was visualized using DAP and methyl green counter stain.

Formalin-fixed, paraffin-embedded human neuroblastoma stained with Exalpha's CD56, clone C5.9 at 2 u..More

Ceramide kinase (CERK) (NT)
(Cat#X2161P)

CERK staining of formallin fixed paraffin embedded human brain (cortex) tissue at a dilution of 20 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

CERK staining of formallin fixed paraffin embedded human brain (cortex) tissue at a dilution of 20 &..More

Ceramide kinase (CERK) (IN)
(Cat#X2160P)

CERK staining of formallin fixed paraffin embedded human brain (cortex) tissue at a dilution of 20 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

CERK staining of formallin fixed paraffin embedded human brain (cortex) tissue at a dilution of 20 &..More

Ceramide Transfer Protein (CERTL)
(Cat#X2379P)

Immunohistochemical staining of normal human liver tissue using CERT antibody (Cat. No. X2379P) at 15 µg/ml

Immunohistochemical staining of normal human liver tissue using CERT antibody (Cat. No. X2379P) at 1..More

chx 10 (Visual system homeobox 2) (CT)
(Cat#X1179P)

Immunohistochemical staining of human melanoma tumor tissue using chx10 (CT) antibody (Cat. No. X1179P).  Antibody used at ~20 µg/ml.

Immunohistochemical staining of human melanoma tumor tissue using chx10 (CT) antibody (Cat. No. X117..More

Corticotropin-releasing hormone receptor 2
(Cat#X2726P)

Staining of normal human colorectal tissue with CRFR1 antibody (Cat. No. X2726P) at 2 µg/ml.

Staining of normal human colorectal tissue with CRFR1 antibody (Cat. No. X2726P) at 2 µg/ml.

Cysteine string protein (CSP) Alpha, DNAJC5 alpha
(Cat#X2667P)

Immunohistochemical staining of human melanoma tissue using affinity purified CSP alpha antibody (Cat. No. X2667P) at 15 µg/ml.

Immunohistochemical staining of human melanoma tissue using affinity purified CSP alpha antibody (Ca..More

Cytochrome P450 1A2
(Cat#X2046M)

Immunohistochemical staining of formalin-fixed, paraffin-embedded normal human liver tissue section.

Immunohistochemical staining of formalin-fixed, paraffin-embedded normal human liver tissue section.

Cytochrome p450 26A1
(Cat#X2049M)

Immunohistochemical staining of liposarcoma cells using CYP26A1 antibody

Immunohistochemical staining of liposarcoma cells using CYP26A1 antibody

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