Custom Services- IgY Production

Schedule
Day	Action	Notes
0	Pre-immunization egg collection	If requested
0	Immunize chickens with 0.25 mg of antigen mixed with Complete Freund's Adjuvant
10	Immunize chickens with 0.15 mg of antigen mixed with Incomplete Freund's Adjuvant
20	Immunize chickens with 0.15 mg of antigen mixed with Incomplete Freund's Adjuvant
30	Immunize chickens with 0.15 mg of antigen mixed with Incomplete Freund's Adjuvant
40-60	Post-immune Egg collection	Depending on the chicken's egg laying habits
Process Details
Antigen requirement	0.7 mg per 1 chicken protocol for immunizations. We also require you to fill out our antigen form with antigen details. You can find our form by clicking on this link.
Average yield	12-15 eggs per chicken
lgY yield	Approximately 60 mg/yolk
Notes	Yolk lgY isolation is available for nominal cost, and can be shipped in PBS containing 0.1% NaN3
We can also ship intact eggs

How much antigen should you ship us?

We recommend immunization with, between 0.5 and 2 milligrams of purified or recombinant protein per hen and prefer a concentration of at least 2 mg/ml. If possible, please supply us with 4 vials of immunogen (for 4 immunizations), the first vial (for the primary immunization) with twice as much antigen as the others.

Where do you ship your antigen?

Ship your purified immunogen, recombinant protein or peptide KLH conjugate to us at:

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

Overview

For those customers requiring large scale production of IgY, we can meet your specific requirements. All laboratory work is performed using standard operating procedures to assure the highest quality of your custom product depending on your immunogen, how much IgY is required and over what time-frame, we will design a custom project that will suit your needs.

For bulk quantities of IgY, we usually recommend multiple hen immunizations and the pooling of their eggs for IgY purification.