Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Phospho-Akt1 ELISA Kit Phospho-Akt1 (S473)

The Exalpha Biologicals, Inc. Phospho Akt1 (pan)(S473) (also known as RAC-PK-alpha (rac), protein kinase B (PKB) or C-AKT) ELISA is a non-isotopic immunoassay for the in vitro quantitation of human/mouse/ rat Phospho Akt1 (S473) protein in cell lysates.

 

Features
- Colorimetric assay
- HTS compatible format
- High sensitivity
- 4 deg C storage with long shelf life
- Non-radioactive
- 5 hour protocol

Performance Summary
- Sensitivity: 100 pg/ml

Ordering Information

Cat. No.
X1844K (pdf)

Size
1 Plate

For larger quantity pricing, please contact Customer Service

Protocol Summary: Not to be used in place of detailed protocol. See kit manual for complete protocol.

Steps
Incubation

1. Equilibrate kit to room temperature

-
2. Add samples and standards to wells 2 hours room temp. (or overnight 4°C)
3. Wash 4x -
4. Add detector antibody to all wells 2 hours - room temp.
5. Wash 4x -
6. Add HRP conjugate to all wells 30 minutes - room temp.
7. Wash 4x -
8. Add substrate to all wells 30 minutes - room temp.
9. Add stop solution to all wells -
10. Read plate at 450 nm/550 nm -