Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Phospho-Akt1 ELISA Kit Phospho-Akt1 (S473)

The Exalpha Biologicals, Inc. Phospho Akt1 (pan)(S473) (also known as RAC-PK-alpha (rac), protein kinase B (PKB) or C-AKT) ELISA is a non-isotopic immunoassay for the in vitro quantitation of human/mouse/ rat Phospho Akt1 (S473) protein in cell lysates.

 

Features
- Colorimetric assay
- HTS compatible format
- High sensitivity
- 4 deg C storage with long shelf life
- Non-radioactive
- 5 hour protocol

Performance Summary
- Sensitivity: 100 pg/ml

Ordering Information

Cat. No.
X1844K (pdf)

Size
1 Plate

For larger quantity pricing, please contact Customer Service

Protocol Summary: Not to be used in place of detailed protocol. See kit manual for complete protocol.

Steps
Incubation

1. Equilibrate kit to room temperature

-
2. Add samples and standards to wells 2 hours room temp. (or overnight 4°C)
3. Wash 4x -
4. Add detector antibody to all wells 2 hours - room temp.
5. Wash 4x -
6. Add HRP conjugate to all wells 30 minutes - room temp.
7. Wash 4x -
8. Add substrate to all wells 30 minutes - room temp.
9. Add stop solution to all wells -
10. Read plate at 450 nm/550 nm -