Accelerating the Pace of Discovery

Comparison of IgG, IgE, IgY and IgY (ΔFc)

IgG IgE IgY IgY (ΔFc)
Species Mammals Mammals Birds, Reptiles, Amphibians Lungfish Anseriform Birds, Amphibians, Lungfish
Molecular Weight (kD) 150 200 180 118
Isoelectric Point (pI) 6.4-9.0 (Ref. 1) 5.2-5.8 (Ref. 2) 5.7-7.6 (Ref. 3) 5.2-7.3 (Ref. 4)
Extinction Coefficient* 1.40 1.62 (Ref. 5) 1.36 1.38
No. Constant Domains 4 4 3 2
Carbohydrate Content (%) 2-4 12 4 (Ref. 6) 0.6 (Ref. 7)
Hinge Region Yes No No No
Antigen Valency 2 2 2 2
Major Serum Antibody Yes No Yes Yes
Source Serum Serum Serum/Egg Yolk Serum/Egg Yolk
Concentration (mg/ml) 10-12 10-4 Chicken: Serum: 8-10 (Ref. 10) Yolk: 10-20 (Ref. 9) Duck: Yolk: IgY (ΔFc): 3-12 (Ref. 4)
Mammalian Complement binding Yes No No No
Rheumatoid Factor Binding Yes No No No
Fc receptor binding Yes No No No
Mediates Anaphylaxis No Yes Yes No
Binding to Protein A Yes No No No
Binding to Protein G Yes No No No
Binding to Protein L Yes Yes No (Ref. 8) No

* optical density of a 1.0 mg/ml solution at 280 nm.

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  2. Clerc, M. -F., Granato, D. A. and M. Horisberger, (1988). Labelling of colloidal gold with IgE. Histochemistry and Cell Biology 89 : 343-349.
  3. Dávalos-Pantoja L, Ortega-Vinuesa JL, Bastos-González D, Hidalgo-Alvarez R, (2000) A comparative study between the adsorption of IgY and IgG on latex particles. J Biomater Sci Polym Ed. 11:657-73.
  4. Chiou, Victor, 2003. Process for selectively isolating IgY antibodies from egg yolk of an anseriform bird and IgY antibodies obtained thereby. United States Patent Application Publication 20060223986.
  5. Liu, F.T., Bohn, J.W., Ferry, E.L., Yamamoto, H., Molinaro, C.A., Sherman, L.A., Klinman, N.R., and Katz, D.H. (1980) Monoclonal dinitrophenyl-specific murine IgE antibody preparation, isolation and characterization. J. Immunol. 124 : 2728-2736.
  6. Ohta M, Hamako J, Yamamoto S, Hatta H, Kim M, Yamamoto T, Oka S, Mizuochi T, Matsuura F., 1991, Structures of asparagine-linked oligosaccharides from hen egg-yolk antibody (IgY). Occurrence of unusual glucosylated oligo-mannose type oligosaccharides in a mature glycoprotein.
  7. Zimmerman, B., Shalatin, N. and H.M. Grey (1971). Structural studies on the duck 5.7S and 7.8S immunoglobulins. Biochemistry 10 : 482-488.
  8. Nilsson, E. Larsson, A. (2005) Chicken Anti-Protein L for the Detection of Small Amounts of Protein L in the Presence of IgG. Hybridoma. 24(2): 112-114
  9. Carlander, D. 2002.Avian IgY Antibody. In vitro and in vivo. Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1119.53 pp. Uppsala. ISBN 91-554-5227-2
  10. Wang, Y.W., Cherian, G., Sunwoo, H.H and Sim. J.S. 2000. Dietary polyunsaturated fatty acids significantly affect laying hen lymphocyte proliferation and immunoglobulin G concentration in serum and egg yolk. Can. J. Anim. Sci. 80:597-604.