Exalpha Biologicals, Inc.

References

1. Seifert, A.W., et al. "Skin shedding and tissue regeneration in African spiny mice (Acomys)." Nature, (2012), 489, 561-565.

2. Fink, K., et al. "The role of metalloproteinases in modification of extracellular matrix in invasive tumor growth, metastasis and angiogenesis." Postepy Hig Med Dosw (Online), (2012), 66, 609-628.

3. Kim, K.S., et al. "Expression levels and association of gelatinases MMP-2 and MMP-9 and collagenases MMP-1 and MMP-13 with VEGF in synovial fluid of patients with arthritis." Rheumatol. Int., (2011), 31, 543-547.

4. Wada, Y., et al. "Diltiazem, a calcium antagonist, inhibits matrix metalloproteinase-1 (tissue collagenase) production and collagenolytic activity in human vascular smooth muscle cells." Int. J. Mol. Med., (2001), 8, 561-566

5. Baici, A., et al. "A handy assay for collagenase using reconstituted fluorescein-labeled collagen fibrils." Anal. Biochem., (1980), 108, 230-232.

'Scar free healing' in mice may give clues to human skin repair, says study

In African spiny mice, the mouse skin's tensile strength was 20 times weaker than in other mice. These mice have brittle skin, which actually tears off in order to escape predators (up to 60% of the skin from their backs). This is useful as a survival trait only if you can grow it back, which it turns out, it does. Unlike wounds in other mammals, the skin then rapidly healed and regrew hairs rather than forming a scar. Research into how these mice regenerate skin may offer clues to healing wounds without scarring, and even regrow limbs or perhaps other more complicated organs.

It's been known for quite some time that salamanders can regrow entire limbs. They are, in fact, famed for their regenerative abilities. Mammals, however, have very limited ability to regrow lost organs. Normally a scar forms to seal the wound. The best humans can do is perhaps regrow the tip of a finger. Scientists have long sought to speed up the healing process. It turns out, slowing it down may be the way to go - as the studies on spiny mice and salamanders show, slowing things down may be the path towards regeneration.(1)

The key may be the formation of a regeneration hub. Tests showed the mice produced a "regeneration hub" or blastema in order to repair the injury. It is this bundle of stem cells that is also used by the salamander to rebuild missing body parts. It is the failure to form a blastema in humans that is one of the main constraints to regenerating appendages.

Another main component of wound healing is repair of the extracellular matrix - or the web of proteins that holds cells in place. Spiny mice appear to deposit extracellular matrix into their wounds at a much slower rate than mice, pigs or humans do and it is perhaps this slower rate that allows time for a blastema to form - slowing things down might be the path towards regeneration.(2,3,4,5)


FITC-Labeled Collagen Substrates
Cat. No.NameProduct TypeSize
X1098Bovine Type I CollagenFITC Conjugate100 Tests (10 mg)
X1099Bovine Type II CollagenFITC Conjugate100 Tests (10 mg)

Collagen and Collagenase related products
Cat. No.NameProduct TypeSize
X2053MMatrix Metalloproteinase 1 (MMP1)Monoclonal Antibody200 µg
X2054MMatrix Metalloproteinase 2 (MMP2)Monoclonal Antibody200 µg
X2056MMatrix Metalloproteinase 9 (MMP9)Monoclonal Antibody200 µg
X2057MMatrix Metalloproteinase 9 (MMP9)Monoclonal Antibody200 µg
X2219PMatrix Metalloproteinase 9 (MMP9)Antigen Affinity Purified Polyclonal50 µg
X2220PMatrix Metalloproteinase 9 (MMP9)Antigen Affinity Purified Polyclonal200 µg