Catalogue

Background
The discovery of NPDC-1 (neuronal proliferation, differentiation and control protein 1) was the result of a study of genes that were preferentially expressed in immortalized neural precursor cell lines at the onset of contact inhibition of proliferation and subsequent differentiation. It was further found that transfection of transformed cells with NPDC-1 led to increased doubling times and suppression of many characteristics of transformation. NPDC-1 is differentially expressed in brain and lung and has been shown to interact directly with the transcription factor E2F-1 as well as D-type cyclins and cdk2. NPDC-1 has been identified as a modulator of transcriptional events mediated by retinoic acid and the protein level and activity of NPDC-1 have been shown to be regulated by phosphorylation mediated proteasomal degradation.

Source
Hybridoma produced by the fusion of splenocytes from mice immunized with a synthetic peptide SLGSPVSSDPVHMS derived from human NPDC-1 protein (Gene ID: 56654) and mouse myeloma cells.

Product

Product Form: Unconjugated

Formulation: 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, pH 7.5, 3mM sodium azide

Purification Method: Ion-exchange chromatography

Concentration: See vial for concentration

Specificity
Monoclonal Anti-NPDC-1 (mouse IgG1 isotype) is derived from the hybridoma 16E4 produced by immunizing mice with the peptide SLGSPVSSDPVHMS (Gene ID: 56654).

Applications
Antibody can be used for Western blotting (1:1000 dilution). Optimal concentration should be evaluated by serial dilutions. Monoclonal Anti-NPDC-1 recognizes human NPDC-1 by immunoblotting (34-40 kDa).

Functional Analysis: Western Blotting

Positive Control: Brain tissue lysate (mouse and rat tested, human not yet tested).

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Spencer, M.L., et al. 'NPDC-1, a novel regulator of neuronal proliferation, is degraded by the ubiquitin/proteasome system through a PEST degradation motif.' J. Biol. Chem. (2004), 279, 37069-37078 2. Evrard, C. & Rouget P. 'Subcellular localization of neural-specific NPDC-1 protein.' J Neurosci Res (2005), 79, 747-755 3. Sansal, I., et al. 'NPDC-1, a regulator of neural cell proliferation and differentiation, interacts with E2F-1, reduces its binding to DNA and modulates its transcriptional activity.' Oncogene (2000), 19, 5000-5009

Protein Reference(s)

Database Name: UniProt

Accession Number: Q8WXX4

Species Accession: Human

Safety Datasheet(s) for this product: