Catalogue

Background
Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining.

Source
Sheep were immunized with bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Applications: Immunoprecipitation: 25-100 µg/ml; Immunohistochemistry: 25-100 µg/ml. The antibody has been tested using immunoprecipitation against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU) or a control (no antigen). At a concentration of 25 ug/ml, this product demonstrates 8-fold higher reactivity with 5-MeC versus BrdU. For best results, use product at 25-100µg/ml. Note: Denature DNA sample first so that bases are accessible to anti BrdU antibody. Applicable in flow cytometry for the quantification of Brdu labeled cells, indication the proliferative activity of the tested cell population.

Functional Analysis: Immunohistochemistry on BrdU-labeled cells

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Nakamura, S., et al. Application of bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody for the in vivo analysis of proliferative characteristics of human leukemia cells in bone marrows. Oncology 1991, 48, 285-289 2. Wilson, G., Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine. Methods Mol. Biol. 1998, 80, 255-266 3. Gray, J. (Ed), Special Issue: Monoclonal antibodies against bromodeoxyuridine. Cytometry 1985, Vol. 6(6)

Protein Reference(s)

Database Name: UniProt

Accession Number: N/A

Safety Datasheet(s) for this product: