Exalpha Biologicals, Inc.

Frontier™ PIG-3 (Human p53 Inducible Gene 3), A Stable Marker for p53 Induction, ELISA Detection Kit (Patent Pending)

Exalpha’s Frontier™ PIG3 Assay is a non-isotopic, non-radioactive immunoassay for the quantitation of the p53 inducible gene 3 (PIG-3) - a long-lived reporter of transient p53 activation.¹ The assay is suitable for human sera, plasma, tissue culture supernatants and cellular extracts or lysates.

1. Flatt, P.M. "p53-dependent expression of PIG3 during proliferation, genotoxic stress, and reversible growth arrest" Cancer Lett 2000, 156, 63-72

Features - High sensitivity - 4 deg C storage with long shelf life - Non-radioactive - 2.5 hour protocol - Suitable for sera, plasma, culture supernatants, cellular extracts/lysates - HTS compatible format	Performance Summary - Sensitivity: 40 pg/ml - Recovery: serum, >98%; plasma, >98% Precision Studies - Intra-assay C.V.'s: <3% mid-level - Inter-assay C.V.'s: <4% mid-level
	Ordering Information
	Cat. No. X1326K1 X1326K2 X1326K3	Size 1 x 96 wells 2 x 96 wells 10 x 96 wells
	For larger quantity pricing, please contact Customer Service

Legend:
Exalpha’s Frontier™ PIG-3 assay. Graphs show pooled coefficients of variation and between assay coefficients of variation (top), senitivity of assay with ng/ml of PIG-3 versus absorbance at 450-550 nm (middle) and standard curve with ng/ml of PIG-3 versus absorbance at 450-550 nm (bottom).

Protocol Summary: Not to be used in place of detailed protocol. See kit manual for complete protocol.

1. Equilibrate kit to room temperature
2. Add samples and standards to wells Add detector antibody to wells	• Incubate 2 hours at room temperature
3. Wash 3 times
4. Add conjugate to all wells	• Incubate 30 minutes at room temperature
5. Wash 3 times
6. Add substrate to all wells	• Incubate 30 minutes at room temperature
7. Add stop solution to all wells
8. Read plate(s) at 450 nm/550 nm